| Instrument | Method | Week 0 | Week 6 | Week 10 | Week 16 | Week 52 |
---|---|---|---|---|---|---|---|
Primary outcomes | |||||||
 Biological aging: telomere length, telomerase activity | Fasting blood samples | Blood | X | – | – | X | X |
Secondary outcomes | |||||||
 Biological and general health indicators | |||||||
  Biomarkers (inflammation, metabolic syndrome) | Fasting blood samples | Blood | X | – | – | X | X |
  Gene-expression (RNA) | Fasting blood samples | Blood | X | – | – | X | X |
  HPA-axis (cortisol) | 2 days of 6 saliva samples | Saliva | X | – | – | X | X |
  Oxidative stress | Urine sample | Urine | X | – | – | X | X |
  Autonomic nervous system | Electro + impedance cardiography, heart rate variability [81] | ME | X | – | – | X | X |
  Blood pressure | Systolic and diastolic BP | ME | X | – | – | X | X |
  Body composition | Weight, height, waist+ hip circumfereence | ME | X | – | – | X | X |
  Physical condition | Astrand sub max test [82] | ME | X |  | – | X | X |
  Muscle strength | Hand grip strength [81] | ME | X | – | – | X | X |
  Lung function | Peak expiratory flow [82] | ME | X | – | – | X | X |
  Pain | Chronic graded pain scale [83] | Int | X | – | – | X | X |
  Somatization | Short somatization questionnaire [84] | SR | X | – | – | X | X |
  Disability severity | WHO-DAS II [85] | SR | X | – | – | X | X |
 Depressive and anxiety disorders | |||||||
  Presence of MDD | CIDI: MDD [78] | Int | X | – | – | X | X |
  Presence of anxiety dis | CIDI: SocPhob, Agora, GAD, PA [78] | Int | X | – | – | X | X |
  Course of symptoms | Life-chart [86] | Int | X | – | – | X | X |
  Severity of depression | Inventory of depressive symptoms [87] | SR | X | X | X | X | X |
  Severity of anxiety | SR | X | X | X | X | X | |
  Sleep | Insomnia Rating Scale [90] | Int | X | – | – | X | X |
 Descriptive variables, potential confounding covariates and potential mediating variables | |||||||
  Age, gender, ethnicity | Standard questions | Int | X | – | – | – | – |
  Socio-economic status | Education, income, occupation | Int | X | – | – | – | – |
  Physical activity | SQUASH questionnaire [91] | SR | X | – | – | X | X |
  Smoking | Past + current smoking questions | SR | X | – | – | – | X |
  Medication use | Drug container observation | Int | X | – | – | X | X |
  Regular alcohol intake | AUDIT questionnaire [92] | SR | X | – | – | – | X |
  Somatic diseases | Presence + symptoms of disease | Int | X | – | – | X | X |
  Health care | Perceived need of care [93] | Int | X | – | – | X | X |
  Work and disability | Tic-P questionnaire [94] | Int | X | – | – | X | X |
  Personality | NEO-FFI questionnaire [95] | SR | X | – | – | X | X |
  Locus of control | Pearlin & Schooler mastery scale [96] | SR | X | – | – | X | X |
  Depression vulnerability | LEIDS-R questionnaire [97] | SR | X | – | – | X | X |
  Anxiety vulnerability | Anxiety senstivity index [98] | SR | X | – | – | X | X |
  Experimental cognitive task | Implicit association test (IAT) [99] | CT | X | – |  | X | X |
  Experimental memory task | Digit Span (WAIS) [100] | Int | X | – | – | X | X |
  Important neg + pos life events | Brugha questionnaire [101] | Int | X | – | – | X | X |
  Childhood Trauma | Youth Trauma questionnaire [102] | SR | X | – | – | – | – |
  Familiy history | Familiy history inventory | Int | X | – | – | – | – |
 Neuroimaging assessment (subsample) | |||||||
  Verbal episodic memory | 15-words test [103] | MRI | x | – | – | x | x |
  Task-related brain activity | Emotional face matching paradigm and N-back paradigm [104] | MRI | x | – | – | x | x |
  Brain network connectivity | Resting state MRI images | MRI | x | – | – | x | x |
 Process indicators (intervention adherence) | |||||||
  Exercise intervention group: Exercise participation and heart rate will be administrated during each session.. | |||||||
   Antidepressant intervention group | Side effect medication questionnaire | Int | – | X | X | X | – |
Adherence (pill count) | Int | – | X | X | X | – |