Table 2 Primers and probes sequences of SNPs
SNP | Primer (5′-3′) | Probe (5′-3′) |
|---|---|---|
rs3088077 | Forward: GGCTGTGTTGCTTGATAGATGTTT | Probe 1: FAM-TTCCAGACCAcGAGCCCCTAGTG--TAMRA |
Reverse: GTCCCCAGCTGCAGACCTT | Probe 2: HEX-TTCCAGACCAtGAGCCCCTAGTGG-TAMRA | |
rs11550869 | Forward: AAAGCACCTTCTGATTGTATAACACTTT | Probe 1: FAM-TCTGGAACTTCTGACAATCTGcCATGA-TAMRA |
Reverse: TACTTAACCTCTCTGGATTCATATTTCTTC | Probe 2: HEX-TCTGGAACTTCTGACAATCTGgCATGA-TAMRA | |
rs7162435 | Forward: TACTGCTTTGTGGATCTTTAATGTTTG | Probe 1: FAM-ACCAATGGTCAAtAGGATATGCAGGCA-TAMRA |
Reverse: GCAATGGGTAAAAAGTATTAAAGCCT | Probe 2: HEX-TGGTCAAcAGGATATGCAGGCAAGA-TAMRA | |
rs2303579 | Forward: TTACTTGACGGTGGAGGTGATG | Probe 1: FAM-AAGGCCTGGcTGC--MGB |
Reverse: TAAGAGAAGATGAAGCCACCATGTA | Probe 2: HEX-AAGGCCTGGtTGCT-MGB | |
rs62043855 | Forward: CAGATGTCCTATGCATGAGCTTAATAT | Probe 1: FAM-CTGAATCAGAATtAAG-MGB |
Reverse: GTGATTGTAAACCAGAAATGTCAGAAA | Probe 2: HEX-CTGAATCAGAATgAAG-MGB |